Table 1.
Crystallographic and Refinement Statistics
| CHIP U-box: UbcH5a complex | |
| Data Collection | |
| Space group | P212121 |
| Unit cell | |
| a, b, c (Å) | 79.0, 93.4, 144.0 |
| α,β,γ (°) | 90, 90, 90 |
| No. molecules/asymmetric unit | 2 CHIP, 2 UbcH5a |
| Resolution (Å) | 55.00-2.90 (3.00-2.90)a |
| Unique reflections | 24283 |
| Completeness (%) | 99.9 (100) |
| Redundancy | 3.40 (3.42) |
| I/σ | 8.5 (1.6) |
| Rmerge(%)b | 8.0 (46.6) |
| Refinement | |
| Resolution (Å) | 53.2-2.90 (3.08-2.90) |
| Rwork(%)c | 24.0 |
| Rfree(%)d | 27.2 |
| Protein atoms | 3518 |
| Waters | 4 |
| Other atoms | 3 |
| Average B factor (Å2) | 65.0 |
| r.m.s.d. – bond lengths (Å) | 0.007 |
| r.m.s.d. – bond angles (°) | 1.4 |
| r.m.s.d. – dihedrals (°) | 23.4 |
| r.m.s.d. – improper angles (°) | 1.1 |
| Ramachandran plot (%) | |
| Most favored and allowed | 97.6 |
| Generously Allowed | 2.4 |
| Disallowed | 0.0 |
| PDB ID code | 2 OXQ |
aStatistics for the highest resolution shell are shown in parentheses.
bRmerge = Σ|Ii-<Ii>|/Σ|Ii|
cRwork = Σh||Fobs(h)|-k|Fcalc(h)||/ΣhFobs(h)
dRfree is the R factor calculated for 5% of reflections, excluded from refinement