Fig. 1.

Cytological analysis of a transgenic plant, G40 (T₁ generation) and its offspring carrying minichromosome α. (A) A metaphase cell of G40 with four different aberrant chromosomes (α, β, γ, and δ) in addition to mini 4S. (B) Schematic representation of chromosome constitution of G40 estimated from A and other FISH images. (C) Mitotic metaphase. (D) Pachytene nucleus of a Tr α plant with a mini α (arrow) as a supernumerary chromosome. The centromere-specific 180-bp repeat, 5S and 18S rDNA, and pBGF101 DNA to detect T-DNA insertion sites were used as probes. (Scale bars: 5 μm.)