Fig. 4.

DNA-fiber FISH and genomic Southern blot analyses. (A) DNA-fiber FISH with chromatin from a Tr α plant and a G40-derived plant carrying β and mini δ chromosomes, probed with T-DNA of pBGF101 (pink) and 180-bp repeats (green). (B) Southern blot analysis of genomic DNA isolated from a Tr α plant, probed with NPTII (neomycin phosphotransferase gene) and GFP (green fluorescence protein gene), M: DNA marker, lanes 1–6: BamHI, EcoRI, EcoRV, HindIII, SmaI, and XbaI digests. (C) A schematic pattern of T-DNA insertions estimated from A and B. (Scale bar: 10 μm.)