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Mapping the interaction regions on BES1 and ELF6/REF6 by GST pull-down assays. (A) Full length and truncated forms of BES1 protein were used to test their interaction with GST-tagged ELF6-C/REF6-C proteins. (B) ELF6-C/REF6-C proteins with serial deletions of zinc finger motifs and MBP-BES1-N were used for GST pull-down assay. All BES1 fragments were fused with MBP and detected with anti-MBP antibody in Western blots.
