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. 2008 May 21;105(21):7546–7551. doi: 10.1073/pnas.0709981105

Fig. 3.

Fig. 3.

Decreasing the FABP5/CRABP-II ratio diverts RA from PPARβ/δ to RAR and inhibits proliferation in cell lines derived from MMTV-neu mice tumors. (a) Immunoblots of CRABP-II and FABP5 in cell lines derived from tumors that arise in MMTV-neu (NaF), MCRABP-II (MTg-II), and MCRABP-II−/− (MII−/−) mice. (b) mRNA for Cyp26a and PDK-1 in MTg-II cells. Cells were treated with 0.1 μM RA for 4 or vehicle. (c) mRNA for Cyp26a and PDK-1 in MIIminus]/− cells and in MII−/− cells ectopically overexpressing CRABP-II (+II). Cells were treated with RA or vehicle. (Inset) Western blot demonstrating ectopic overexpression of CRABP-II in transfected cells. (d) BrdU incorporation assays in denoted cell lines and in cell lines transfected with FABP5 siRNA (5si). Cells were treated with 1 μM RA for 24 h or vehicle. *, P = 0.001; **, P = 0.02 versus corresponding untreated controls. (e) Cell-cycle distribution. Denoted cells were treated with vehicle or 1 μM RA for 5 days, fixed in ethanol, stained with propidium iodide, and analyzed by FACS.