Fig. 4.

Decreasing the FABP5/CRABP-II ratio triggers apoptosis and differentiation in cell lines derived from MMTV-neu mice tumors and suppresses tumor growth in vivo. (a) Denoted cell lines were treated with 1 μM RA for 12 h or 10 ng/ml TRAIL (TR) for 12 h. Cells were lysed and the level of cleaved caspase 3 (cl. casp. 3) was assessed by immunoblots. (b) Quantitation of data in a. Values are mean ± SEM (n = 3). *, P = 0.05 versus untreated II^−/− cells. (c) Denoted cells were stained with Oil-red-O 2 days postconfluence. (d) β-casein mRNA in MTg-CRABP-II cells preconfluence and 1.5 days postconfluence. Cells were treated with 1 μM RA throughout the experiment. *, P = 0.04 versus nontreated confluent MTg-II cells. (e) Tumor growth in MMTV-neu (Mneu), MTg-CRABP-II (MTg-II), and M-CRABP-II^−/− (M-II^−/−) mice. Tumor sizes were measured with a start point (day 0) of tumor volume = 0.065 cm³.