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X-band EPR spectra of as-isolated Mn-MndD (A), as-isolated Mn-HPCD (B), the Mn-MndD–HPCA complex (C), and the Mn-HPCD–HPCA complex (D). Each sample contained ≈250 μM enzyme in 50 mM phosphate buffer (pH 8). 1 mM HPCA was present in the substrate complexes. EPR parameters: 4 K, 1-mW microwave power at 9.35 GHz, modulation amplitude 10 G.
