. 2008 May 20;105(21):7347–7352. doi: 10.1073/pnas.0711179105

Table 1.

Steady-state kinetics and metal content of HPCDs and MndDs

Enzyme	Metal	[M]/[enzyme]^*		K_M^HPCA, μM	K_M^O2, μM	k_cat, min⁻¹	k_cat/K_M^HPCA, μM⁻¹·min⁻¹	k_cat/K_M^O2, μM⁻¹·min⁻¹
Enzyme	Metal	Fe	Mn	K_M^HPCA, μM	K_M^O2, μM	k_cat, min⁻¹	k_cat/K_M^HPCA, μM⁻¹·min⁻¹	k_cat/K_M^O2, μM⁻¹·min⁻¹
HPCD	Fe(II)	0.7	<0.1	31 ± 6	60^†	470 ± 20	15 ± 2	7.8 ± 0.3
	Mn(II)	<0.1	0.8	35 ± 5	50 ± 4	370 ± 10	11 ± 2	7.4 ± 0.6
MndD	Mn(II)	<0.1	0.7	14 ± 4	62^‡	360 ± 20	25 ± 4	5.8 ± 0.3
	Fe(II)^§	0.6	<0.1	15 ± 4	37 ± 6	420 ± 20	28 ± 4	11.4 ± 1.4
	Fe(III)^¶	0.6	<0.1	15 ± 4	37 ± 6	29 ± 2	2 ± 1	0.8 ± 0.4

*ICP analysis. Enzyme is defined in moles of metal/moles of monomer.

^†From ref. 14.

^‡From ref. 13.

^§After reactivation with ascorbate.

^¶After exposure to air for 1 h.