Fig. 3.

Ash2l increases Ap2δ transactivation in a dose-dependent manner. 293T cells were transfected with a luciferase reporter under the control of GAL4 UAS and a minimal promoter and varying concentrations of GAL4 DBD or Ap2δ N terminus fused to a GAL4 DBD; amounts of those constructs transfected are indicated below the graph. In addition, varying amounts of an expression construct for Ash2l were cotransfected; the shading of the bars corresponds to the dose (indicated in the key to the right of the graph). The heights of the bars represent means of ratios of the reporter luciferase activity to the control Renilla luciferase [relative light units (RLU)] for each condition. Results are expressed as means ± SEM and compared statistically, using Student's t tests. **, P < 0.005; ***, P < 0.0001.