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ITC measurement of the binding of the MLL CXXC domain to CpG1II oligonucleotide. Data shown is for addition of 10-μl aliquots of 550 μM CXXC domain to a 46 μM solution of CpG1II. The data were fit to a one-site binding model, giving Kd = 7.8 ± 1.4 μM and n = 0.93 ± 0.04.
