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. 2008 May 20;105(21):7393–7398. doi: 10.1073/pnas.0802981105

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© 2008 by The National Academy of Sciences of the USA

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Fig. 2. — LC-MS analysis of reticuline produced in E. coli (A) or (S)-reticuline synthesized in vitro (B). The selected ion monitoring (SIM) parameters are as follows: m/z = 153 (3,4-DHPAA), m/z = 154 (dopamine), m/z = 288 (norlaudanosoline), m/z = 302 (3′-hydroxycoclaurine), m/z = 316 (3′-hydroxy-N-methylcoclaurine), m/z = 330 (reticuline). The inset shows the time course of reticuline production in E. coli (A) or (S)-reticuline synthesized in vitro (B). Error bars represent the SD of three independent measurements.