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. 2008 May 20;105(21):7393–7398. doi: 10.1073/pnas.0802981105

Fig. 4.

Fig. 4.

LC-MS analysis of magnoflorine (A) or scoulerine (B) produced in mixed culture of microbes. SIM parameters are as follows: m/z = 153 (3,4-DHPAA), m/z = 154 (dopamine), m/z = 288 (norlaudanosoline), m/z = 302 (3′-hydroxycoclaurine), m/z = 316 (3′-hydroxy-N-methylcoclaurine), m/z = 328 (corytuberine or scoulerine), m/z = 330 (reticuline), m/z = 342 (magnoflorine or N-methylscoulerine). The inset shows the time course of magnoflorine or scoulerine/N-methylscoulerine production in microbes. E. coli culture, which produced reticuline, was mixed with S. cerevisiae with CYP80G2-CNMT (A) or BBE (B) and cocultured. Cultures were sampled at various intervals and production levels were quantified. The amount of N-methylscoulerine production was calculated by using scoulerine as a standard. The error bars represent the standard deviation of three independent measurements.