Figure 2.

Immunoblots of membrane fractions of inner medulla (IM) and outer medulla plus cortex (OM+C) in the kidneys from Brattleboro rats: CTR (n = 4) and SR (n = 4) rats, protocol 1. Immunoblots were reacted with affinity-purified anti-AQP2 (A and B) and anti-NKCC2 (Na-K-2Cl Cotransporter) (C) antibodies. Densitometric analysis revealed that in the SR-treated Brattleboro rats the expression levels of AQP2 in the IM and OM+C and NKCC-2 in the OM+C were decreased significantly compared with CTR Brattleboro rats.