Figure 1.

Time course of glomerular Vn and endogenous PAI-1 staining in diabetic db/db mice. Representative photomicrographs of glomeruli were from three mice at different ages. Polyclonal rabbit anti-mouse Vn antibody (1:300 dilution; provided by Emile de Heer, Department of Nephrology and Pathology, Leiden University Medical Center, Leiden, Netherlands) and rabbit anti-rat PAI-1 antibody (400 μg/ml dilution; American Diagnostica, Greenwich, CT) were used as the primary antibodies. FITC-conjugated swine anti-rabbit IgG (Dako Corp., Carpinteria, CA) was used as secondary antibody. Control slides treated with PBS instead of primary antibodies showed no staining. Data are from three mice at each group. *P < 0.05 versus uninephrectomized nondiabetic db/m control at 22 wk of age; **P < 0.05 versus uninephrectomized diabetic db/db mice at 18 wk of age. Magnification, ×400.