Figure 2.

Upregulation of PN-1 mRNA in mesangial cells of TSLP transgenic and TSLP/FcγIIb−/− mice. WT mice demonstrate little expression of PN-1 mRNA in glomeruli (A), with increased expression in TSLP transgenic mice (B), and greatest expression in glomeruli of TSLP/FcIIb−/− mice (C). Negative control hybridized with sense probe (D). In both TSLP mice (data not shown) and TSLP/FcIIb−/− mice (E through G), most of the cells immunolabeled with α-SMA also expressed PN-1 mRNA (E), whereas Mac-2+ monocyte/macrophages expressing PN-1 were a small subset of overall PN-1–expressing cells (F and G, arrow). (G) Negative control hybridized with the sense probe. Magnification, ×400.