Figure 3.

Mesangial expression of PN-1, tPA, and PAI-1 proteins were increased in TSLP transgenic and TSLP/FcγIIb−/− mice. PN-1 is localized to podocytes in WT mice (A), and de novo mesangial expression is present in TSLP transgenic mice (B) and further upregulated in TSLP/FcIIb −/− mice (C). Mesangial expression of tPA protein was increased in TSLP transgenic mice (E) compared with WT mice (D) and was even more pronounced in TSLP/FcIIb−/− mice (F). Focal PAI-1 protein expression in WT mice was present in mesangial areas (G), which was upregulated in TSLP transgenic mice (H) and more so in TSLP/FcIIb−/− mice (I). Magnification, ×400.