Figure 4.

Upregulation of PN-1 RNA in mesangial cells exposed to cryoglobulins. (A) Northern blot analysis. Mouse mesangial cells were incubated with cryoglobulin or mouse IgG (control Ig) for the indicated time. RNA extracted from each was analyzed by Northern blot (10 μg/lane) with a cDNA probe specific for PN-1. (B) PN-1 RNA expression. Mouse mesangial cells incubated with cryoglobulins demonstrate upregulated PN-1 RNA expression, increasing of 2.6-fold over control at 24 h. Upregulation became apparent at 6 h and peaked at 24 h. Mesangial cells incubated with control mouse IgG showed no significant change of PN-1 RNA expression. Values are normalized for differences in RNA loading.