Figure 6.

Immunohistochemistry shows that CD3+ T cell infiltration within the kidney with anti-GBM crescentic GN at day 14 is markedly inhibited in TGF-β1 Tg mice. (A, B) Normal Wt (A) and Tg (B) kidneys stained with the anti-CD3 antibody. (C, D) Diseased kidneys from Wt (C) and Tg (D) mice stained with the anti-CD3 antibody. Note that extensive CD3+ T cell infiltration within the diseased kidney in Wt mice (C) contributes significantly to severe glomerular and tubulointerstitial damage, resulting in focal tubulointerstitial damage (*). In contrast, CD3+ T cell infiltration within the diseased kidney in Tg mice is inhibited (D). (E, F) Semiquantitative analysis. Each bar represents the mean ± SEM for a group of eight mice with anti-GBM GN and six normal mice. *P < 0.05, ***P < 0.001 compared with normal mice; ### P < 0.001 when compared with the Wt mice. Magnification, ×200.