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. Author manuscript; available in PMC: 2008 May 28.

Published in final edited form as: Virology. 2007 Feb 6;362(2):374–383. doi: 10.1016/j.virol.2006.11.026

Fig.3 — Demonstration of the effect of NS1 and NS3 mutations on recombinant virus focus formation on monolayers of C7/10 and BHK cells. (A) Schematic representation of the genome of DV1 showing all of the amino acid coding differences between DV1-BR/90 and our BAC plasmid encoded genomes. The position of amino acid residues of NS1 and NS3 that differed between these genomes are shown at the top; bold type has been used to designate the amino acids of consensus sequence in DV1 (see text and Table 1). (B) Photographs of monolayers C7/10 and BHK cells transfected with Lipofectin using similar amounts of synthetic RNA (20 ngs) from the indicated BAC clones, overlaid with semi-solid media, and stained with anti-NS1 antibody 4 days after transfection. (C) Photographs of monolayers C7/10 and Vero infected with DV1-BR/90 or DV1flc, overlaid with semi-solid media, and fixed and immunostained with anti-NS1 antibody 4 days (C/710) or 5 days (Vero) after incubation at 30°C (C7/10) or 37°C (Vero).

Fig.3 — Demonstration of the effect of NS1 and NS3 mutations on recombinant virus focus formation on monolayers of C7/10 and BHK cells. (A) Schematic representation of the genome of DV1 showing all of the amino acid coding differences between DV1-BR/90 and our BAC plasmid encoded genomes. The position of amino acid residues of NS1 and NS3 that differed between these genomes are shown at the top; bold type has been used to designate the amino acids of consensus sequence in DV1 (see text and Table 1). (B) Photographs of monolayers C7/10 and BHK cells transfected with Lipofectin using similar amounts of synthetic RNA (20 ngs) from the indicated BAC clones, overlaid with semi-solid media, and stained with anti-NS1 antibody 4 days after transfection. (C) Photographs of monolayers C7/10 and Vero infected with DV1-BR/90 or DV1flc, overlaid with semi-solid media, and fixed and immunostained with anti-NS1 antibody 4 days (C/710) or 5 days (Vero) after incubation at 30°C (C7/10) or 37°C (Vero).