Fig.7.

Effect of NS1 mutation on DV1 RNA replication. BHK cells were electroporated with identical amount of Rluc-expressing DV1 subgenomic replicon RNAs (with or without the NS1 position 211 mutation), seeded in multi-well plates, and incubated at 30°C or 37°C prior to harvesting lysates for the determination of Rluc activity (see Methods). Rluc activities were measured at 6 hr and 4 days postelectroporation. The Rluc activity obtained at 4 days are shown normalized to the activity obtained at 6 hr, to account for slight differences in transfection efficiency (see text). Data from each condition are shown as an average of triplicate values with error bars showing standard deviations.