Skip to main content
. Author manuscript; available in PMC: 2008 May 28.
Published in final edited form as: Virology. 2007 Feb 6;362(2):374–383. doi: 10.1016/j.virol.2006.11.026

Fig.8.

Fig.8

Affect of NS1 mutation on NS1 synthesis and secretion. Western blots prepared from samples of cell lysates and supernatant fluids obtained from Vero cells infected at a MOI of 0.01 ffu with either DV1flcNS1* and DV1flc viruses (or mock-infected), incubated for 5 days at 30°C (to allow both viruses to infect the entire monolayer), and then shifted to 37°C for 2 days. The media were changed three times with serum free medium one day before harvest to insure that the NS1 protein analyzed represented protein produced at the final incubation temperature. (A) Replica of Western blot showing the NS1 protein (detected with MAb D2-7E11) present in cell lysates. (B) Replica of Western showing the NS1 protein released from the indicated cell monolayers at 37°C, following electrophoresis after denaturation in the presence or absence (to preserve dimers) of heating, and detection with MAb D2-7E11.