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. 2008 Jun 2;181(5):831–846. doi: 10.1083/jcb.200711167

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© 2008 Leal-Ortiz et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Synapsin1a dispersion and FM destaining are tightly correlated. (A and B) FM5-95 destaining curves for individual EGFP-Synapsin1a puncta at boutons with (A) or without (B) Piccolo. Shown in black are examples of FM destaining curves for which there was either dramatic (>40%; triangles) or minimal (<10%; squares) dispersion of Synapsin. Shown in light gray are EGFP-Synapsin intensities for these boutons. (C and D) Correlation analysis of EGFP-Synapsin1a dispersion and FM5-95 destaining at synapses with (C) or without (D) Piccolo. Plotted are EGFP-Synapsin1a and FM intensity values (expressed as a percentage initial fluorescence) at the final time point (t =180 s) of 5-Hz stimulation (control = 709 puncta; Pclo28 = 855 puncta). Dashed lines and circles indicate mean intensity values for Synapsin and FM at control boutons (blue; 90.8 ± 1.0% and 75.6 ± 0.9%, respectively) and those lacking Piccolo (red; 79.2 ± 0.9% and 65.5 ± 0.8%, respectively). Note that values for boutons lacking Piccolo are significantly shifted toward the bottom right.