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. 2008 Jun 2;181(5):791–801. doi: 10.1083/jcb.200711021

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© 2008 Chen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Dysbindin-deficient chromaffin cells display slow release kinetics and reduced depolarization-induced secretion. (A) A typical amperometric recording in response to a 20-s stimulus of 80 mM K⁺ in a WT chromaffin cell. Inset shows a micrograph of a microcarbon fiber electrode (black bar) attached to a chromaffin cell in an adrenal slice during amperometric recording. (B) Averaged traces showing the change in the shape of amperometric spikes in dysbindin-deficient (sdy) mice versus WT mice. (C, left) Three kinetics variables, i.e., HHD, RT, and Q, are defined. (rightmost three panels) Quantitative analyses of HHD, RT, and Q of amperometric spikes from WT (n = 166 events, 11 cells) and sdy mice (n = 131 events, 10 cells). (D) Examples of amperometric current traces (I_amp, top), integrated current signal (∫I_ampdt), and membrane current traces (I_m) evoked by a 2-s depolarizing pulse from −70 to +0 mV in a WT (gray) and a sdy (black) chromaffin cell. (inset) A micrograph of combined patch-clamp and amperometric recording in a chromaffin cell. (E) Histograms show the amount of secretion (integral of amperometric signal), the number of amperometric spikes, and the amplitude of the voltage-gated Ca²⁺ and Na⁺ currents of both cell types. I_Ca = 324.5 ± 34.6 pA (WT) and 339 ± 40 pA (sdy). I_Na = 3.0 ± 0.2 nA (WT) and 2.9 ± 0.2 nA (sdy). Data from 19 WT cells and 29 sdy cells are shown. *, P < 0.05; ***, P < 0.001. Error bars indicate the mean ± SEM. Bars, 10 μm.