Fig. 4.
EGCG increases gene expression of GCLc but not GCLm in activated HSCs in vitro. Passaged HSCs were treated with EGCG at various concentrations for 24 h. Total RNA or whole-cell extracts were prepared for real-time PCR (A) or for Western blotting analyses (B), respectively. GAPDH or β-actin was used as an invariant internal control, respectively. Values are means ± S.D. (n = 3). *, p < 0.05 versus the control cells treated with no EGCG. Representative was shown from three independent experiments.