Table 3.

Primer Sequence

Gene	Primer	Sequence(5′-3′)	Product Length (bp)	Tm (°C)
Myocardin	Forward	GAGAGGTCCATTCCAACTGCTCAGATGAAG	249	62
	Reverse	GTCTTCACTTCGAGTCTGATCCGGAGAAAG
Calponin	Forward	AGCATGTCCTCTGCTCACTTCAACC	154	60
	Reverse	CGTCCATGAAGTTGTTGCCGAT
SM22 α	Forward	TGGGTGGTGATCCACTGGATC	179	59
	Reverse	TTGCCTTGAGTCAGTGCGCC
α−SMA	Forward	AGCCCAGCCAAGCACTGTCA	130	62
	Reverse	GAGCATCGTCCCCAGCAAAG
Myosin heavy chain	Forward	GAAGATCGTCGACATGTACAAGGG	122	57
	Reverse	TGCATAGAATGGACTGGTCCTCC
Matrix Gla protein	Forward	GCTACACAAGACCCTGAGACTGACC	192	61
	Reverse	TCTCCATCTCTGCTGAGGGGAT
Cbfa1	Forward	CACGACAACCGCACCATGGT	181	61
	Reverse	TGACAGTAACCACAGTCCCATCTG
MSX2	Forward	GCTGGTGAAGCCCTTCGAGA	173	59
	Reverse	ATGTGGTAAAGGGCGTGCG
Osterix	Forward	AGTTCATGGCTCCAGTCCCC	146	60
	Reverse	TGGAGGCTGAAAGGTCACTGC
BMP-2	Forward	AGCCAACACTGTGCGCAGCT	140	63
	Reverse	CCTGAAGCTCTGCTGAGGTGATAA
Osteocalcin	Forward	ATGAGAGCCCTCACACTCCTC	297	60
	Reverse	GCCGTAGAAGCGCCGATAGGC
GAPDH	Forward	TCCTGTTCGACAGTCAGCCG	122	60
	Reverse	TGGTGACCAGGCGCCCAATA
Mouse osterix	Forward	TCCCTTCTCAAGCACCAATGGACT	230	60
	Reverse	AGCTGTGAATGGGCTTCTTCCTCA
Mouse GAPDH	Forward	TGTTCCAGTATGACTCCACTCACG	170	60
	Reverse	GAAGACACCAGTAGACTCCACGACA
Mouse Cbfa1	Forward	TGCAAGAAGGCTCTGGCGT	200	60
	Reverse	CGCTGAAGAGGCTGTTTGACG
Mouse MSX2	Forward	ACCACGTCCCAGCTTCTAGC	183	60
	Reverse	GCTCTGCGATGGAGAGGTACTG
Mouse osteocalcin	Forward	TCATGTCCAAGCAGGAGGGCAATA	292	58
	Reverse	TGACATCCATACTTGCAGGGCAGA