Figure 2.

T cells and activated Mø increase during NSN, whereas TEC are a primary source of CXCL9 and CXCL10 during NSN (day 14). (A) Expression of CXCR3 and its ligands CXCL10, CXCL9, and CXCL11 during NSN were evaluated by real-time PCR. (B) The frequency of intrarenal T cells (CD4⁺ and CD8⁺) and Mø expressing CXCR3. Note representative FACS plots of CXCR3 expression on T cells and Mø in WT and control CXCR3^−/− mice during NSN. (C) The frequency of cultured BMMø expressing CXCR3 rises in response to stimulation with LPS. To verify staining specificity, we analyzed CXCR3^−/− BMMø stimulated with LPS and B6 BMMø stimulated with LPS and stained with an isotype control. These data are representative of three samples and two additional experiments that used isotype controls alone. (D) Intrarenal activated Mø increased during NSN. We determined the total number of intrarenal activated (CD86⁺) CXCR3⁺ Mø by multiplying the frequency by the total number of Mø. (E) CXCL9 and CXCL10 are mainly expressed by TEC during NSN (n = 4/group). Intrarenal CXCL9⁺ CD4⁺ and CD68⁺ cells and CXCL10⁺ CD4⁺, CD8⁺, and CD68⁺ cells increase during NSN. We analyzed 0.5 to 1 × 10⁶ cells (B, D, and E) and 2.5 × 10⁵ cells (C) by FACS analysis. Data are means ± SEM.