Figure 4.

Intrarenal activated T cells and activated Mø are suppressed in CXCR3^−/− and CXCL9^−/− mice as compared with WT mice during NSN (day 14). (A) The number of CD4⁺ and CD8⁺ T cells and Mø detected by immunostaining is diminished within glomeruli (intra-, periglomerular) and in the interstitium in CXCR3^−/− and CXCL9^−/− mice as compared with WT mice during NSN. (B) We detected a suppression of activated (CD69⁺) CD4⁺ and CD8⁺ T cells and activated (CD86⁺) Mø in CXCR3^−/− (top) and CXCL9^−/− (bottom) mice as compared with WT mice during NSN. We analyzed 0.5 to 1.0 × 10⁶ cells by FACS (n = 6 to 9/group). (C) We evaluated kidneys from CXCR3^−/− and WT mice for the presence of activated Mø (CD68⁺, TNF-α⁺) during NSN using immunofluorescence. Representative photomicrographs illustrate CD68⁺ cells expressing TNF-α⁺ (circled) in the CXCR3^−/− compared with WT kidneys. Data are means ± SEM; n = 4/group.