Table 1.
Resting [Ca2+]i (nM) levels among subpopulations of DRG neurons from naïve and inflamed rats
| Subpop. | Stats | DiI+ | Stats | DiI− | ||
|---|---|---|---|---|---|---|
| Naïve (n) | CFA (n) | Naïve (n) | CFA (n) | |||
| Small | S < 0.01
C > 0.05 I < 0.01 |
138 ± 8 (20) | 144 ± 8 (19) | S > 0.05
C < 0.05 I > 0.05 |
122 ± 4 (72) | 138 ± 6 (72) |
| Medium | 114 ± 6 (25) | 145 ± 6 (42) | 120 ± 5 (38) | 135 ± 7 (33) | ||
| Large | 121 ± 8 (18) | 102 ± 8 (12) | 118 ± 4 (39) | 125 ± 5 (31) | ||
| IB4+ | S < 0.01
C > 0.05 I < 0.01 |
125 ± 5 (33) | 150 ± 5 (48) | S > 0.05
C < 0.01 I > 0.05 |
120 ± 3 (81) | 140 ± 6 (72) |
| IB4− | 123 ± 7 (30) | 114 ± 6 (25) | 120 ± 4 (68) | 128 ± 4 (64) | ||
| CAP+ | S > 0.05
C > 0.05 I > 0.05 |
128 ± 5 (36) | 141 ± 5 (56) | S > 0.05
C < 0.05 I > 0.05 |
121 ± 3 (74) | 139 ± 5 (80) |
| CAP− | 118 ± 7 (27) | 126 ± 10 (17) | 120 ± 3 (75) | 128 ± 5 (56) | ||
Small, Medium and Large are cell body diameter. IB4 is isolectin B4. CAP is capsaicin. Data are mean ± SEM. The number in parentheses is the number of neurons studied in each group. Two-way ANOVA was used to assess the presence of main effects associated with subpopulation (S: cell body diameter, IB4 binding or CAP sensitivity) and inflammation (C: Naïve versus CFA) as well as a significant interaction (I) between the two. DiI+ and DiI− neurons were analyzed separately. Results of this analysis are indicated in columns labeled Stats. Post-hoc analysis (Holm-Sidak test) indicated that resting [Ca2+]i was significantly (p < 0.05) higher medium diameter DiI+ neurons from inflamed rats than in those from naïve rats. Similarly, post-hoc analysis indicated that resting [Ca2+]i was significantly (p < 0.01) higher medium diameter DiI+ neurons from inflamed rats than in those from naïve rats.