Fig. 5.
IL-10-producing T regulatory cell phenotype in draining and non-draining lymph nodes after facial nerve axotomy. Cells were prepared for FACS analysis as described in Fig. 1. (a) The number of IL10+ CD4+ T cells within the draining RCLN and non-draining MLN were calculated and the percentage is represented by the number within the lower right FACS plot. (b) MFI indicates the level of IL-10 expression in cells from the RCLN. The figure shows representative data of flow cytometry analysis from cells pooled from four animals per group from three independent experiments.