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. Author manuscript; available in PMC: 2009 Feb 28.
Published in final edited form as: J Mol Biol. 2007 Nov 29;376(4):1034–1047. doi: 10.1016/j.jmb.2007.11.064

Table 3.

Parameters from the analysis of the [urea] and [KCl] upshifts at 37 and 10 °C

37 °C 10 °C

k−2 (s−1) 3.3 ± 0.7 7.2 (±0.7) × 10−1

K3o a 2.7 (±0.9) × 105 3.2 (±0.6) × 103

dlnK3/d[urea] b −3.3 ± 0.1 M−1 −3.5 ± 0.1 M−1

k−3o (s−1) a c 1.1 (±0.7) × 10−2 3.3 (±1.0) × 10−2

k3o (s−1) a d 3.0 (±2.2) × 103 1.0 (±0.4) × 102

dlnk−3/d[urea] c 1.1 ± 0.2 M−1

dlnk3/d[urea] e −2.2 ± 0.2 M−1 −2.5 ± 0.2 M−1
a

Xo is the value of X in DB (containing 0.12 M KCl and no urea)

b

Determined from a fit of the linear regions of the data in Fig 3a (0–1.5 M urea at 10 °C and 0–2.5 M urea at 37 °C)

c

Calculated from fits of the data in Figure 3a to eq 5 in the text

d

Calculated from values of K3 (= k3/k−3) and k−3 in this table

e

Calculated from: dlnk3/d[urea] = dlnK3/d[urea] + dlnk−3/d[urea]