Figure 3.

Nuclear accumulation of Flp1p depends on checkpoint kinase Cds1p (A) Flp1p-GFP localization and Hoechst costaining in untreated and 12 mM HU treated flp1-GFP cells. (B) Flp1p-GFP localizes normally in both Δcds1 and Δchk1 untreated cells. (C) Flp1p-GFP localization in 12 mM HU-treated cells lacking either Δcds1 or Δchk1. Whereas Δchk1 cells localize Flp1p-GFP to the nucleus in response to replication stress like wild-type cells, the phosphatase remains nucleolar in cells deleted for cds1. Nuclei magnified 3× are shown. Bar, 4 μm.