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Biochemical properties of SCPL-1. SCPL-1b was expressed as an MBP fusion protein in E. coli, and they are shown to have phosphatase activity toward a model substrate, p-nitrophenyl phosphate (A). Enzymatic properties were very similar to previously characterized FCPs and SCPs, including a pH optimum of ∼5.0 (B), preference for Mg+2 (C), and unusual response to small-molecule inhibitors (not inhibited by NaF or Na3VO4 but inhibited by BeF3− and AlF4; shown in D).
