Figure 5.

HOPS does not require the N-terminal H_ABC domain of Vam3p for 0-layer proofreading activity. Vacuoles from BJ3505 and DKY6281 derivatives bearing Vam3p deleted for its conserved N-terminal H_ABC domain (Vam3ΔN; Laage and Ungermann, 2001) were assayed for fusion under standard ATP-free conditions after 90 min at 27°C. Vam7p proteins were added at the indicated concentration, and HOPS was added to a final concentration of 25.0 nM. Results are the mean ± SD of five independent experiments.