Figure 2.

Changes in Spo13 and Spo2 abundance during meiosis. (A) Cells carrying spo13-HA (YN98) (A) or spo2-HA (YN463) (B) were allowed to proceed through synchronous meiosis. Aliquots were removed at hourly intervals and protein extracts were subjected to Western blot analysis with the mouse anti-HA antibody 12CA5 and with anti-α-tubulin antibody TAT-1 as the loading control. Meiotic nuclear division was monitored by counting the number of nuclei per cell. □, mononucleate; ○, binucleate; ▴, tri- or tetranucleate cells.