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. 2008 Jun;19(6):2476–2487. doi: 10.1091/mbc.E08-02-0118

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© 2008 by The American Society for Cell Biology

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Figure 4. — Magnified and merged images of Spo13 and Spo2 at the SPB. (A) Double staining of SPB components. Strain TN8 carrying pAL(spo13-GFP) (left) and strain YN433 (middle) were induced to undergo meiosis. Cells were fixed and stained with DAPI. Fluorescent images created by use of DAPI, GFP, and the anti-Sad1 antibody were observed under a fluorescence microscope. Blue, DAPI; red, Sad1; green, GFP signal. Right, strain TN29 carrying pAL(Spo13-HA) and pAU(Spo2-GFP) was examined as described above. (B) Double staining of SPB components and the FSM. Left, magnified and merged images of wild-type (YN68) cells harboring integrated GFP-Psy1 during formation of the FSM. Blue, DAPI; red, Sad1; green, GFP-Psy1. Right, magnified and merged images of wild-type (YN114) cells harboring integrated GFP-Psy1 and Spo13-HA. Images of YN114 cells were obtained as described for YN68. Blue, DAPI; red, Spo13-HA; green, GFP-Psy1.