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Localization of ectopically expressed Spo13 and Spo2 during vegetative growth. TN104 cells harboring pREP41(spo13-GFP) together with either pREP1A [control], pREP1A(spo2) [Spo2op], or pREP1A(spo15) [Spo15op] were incubated in MM+N medium at 28°C for 16 h. YN47 (spo15Δ) cells harboring pREP41(spo13-GFP) and pREP1A(spo2) were incubated similarly. Fixed cells were examined after staining by DAPI and GFP, and with the anti-Sad1 antibody.
