Table 2.
Plasmids used in this study
| Plasmid | Characteristics | Source |
|---|---|---|
| pDB248′ | 2 μm origin, LEU2-based vector | Beach and Nurse (1981) |
| pREP1 | ars1, LEU2-based expression vector carrying nmt1+ promoter | Maundrell (1993) |
| pREP2 | ars1, ura4+-based expression vector carrying nmt1+ promoter | Maundrell (1993) |
| pREP41 | ars1, LEU2-based expression vector carrying nmt41+ promoter | Maundrell (1993) |
| pREP42 | ars1, ura4+-based expression vector carrying nmt41+ promoter | Maundrell (1993) |
| pREP1A | derivative of pREP1 that contains ade6+ as selectable marker | T. Tamai (unpublished data) |
| pREP1 (mei4) | pREP1, mei4+-coding region | Abe and Shimoda (2000) |
| pBR (leu1) (FYP1248)a | leu1+ in pBR322 | Nakamura-Kubo et al. (2003) |
| pIL-HA | LEU2-based integration vector, HA-epitope-coding region | This study |
| pAL-KS | ars1, LEU2-based multicopy shuttle vector | Tanaka et al. (2001) |
| pAU-KS | ars1, URA3-based multicopy shuttle vector | Tanaka et al. (2001) |
| pTN143 (FYP410)a | pAL-KS, GFP and nmt1+ terminator | Ikemoto et al. (2000) |
| pREP41 (GFP) | pREP41, GFP | This study |
| pAL (spo13-GFP) | pTN143, spo13-GFP | This study |
| pAL (spo13-HA) | pTN144, spo13-HA | This study |
| pAL (spo13B82-GFP) | pTN143, spo13B82-GFP | This study |
| pREP1A (spo2) | pREP1A, spo2-containing PCR fragment | This study |
| pREP1A (spo15) | pREP1A, spo15, spo13-containing PCR fragment | This study |
| pREP41 (spo13-GFP) | pREP41, spo13-GFP | This study |
| pREP41 (spo2-GFP) | pREP41, spo2-GFP | This study |
| pREP42 (GST-spo2) | pREP42, GST-spo2 | This study |
| pREP42 (GST) | pREP42, Glutathione-S-transferase (GST) | This study |
| pAU (spo2-GFP) | pAU, spo2-GFP | This study |
| pBR (leu1) (spo13-HA) | pBR(leu1), spo13-HA | This study |
| pBR (leu1) (spo2-HA) | pBR(leu1), spo2-HA | This study |
| pBR (leu1) (spo2-GFP) | pBR(leu1), spo2-GFP | This study |
The plasmids constructed in this study will be deposited at the YGRC/NBRP.
a This plasmid was obtained from the Yeast Genetic Resource Center of Japan supported by the National BioResource Project (YGRC/NBRP) (http://yeast.lab.nig.ac.jp/nig/).