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. 2008 Jun;19(6):2620–2630. doi: 10.1091/mbc.E07-07-0674

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© 2008 by The American Society for Cell Biology

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Figure 4. — Association of p97 with ERdj4, ERdj5, and SP-C. (A) HEK293-SP-C^WT and HEK293-SP-C^Δexon4 (x4) stable cell lines were treated with MG-132 and cross-linked with DSP. Cell lysates were immunoprecipitated with p97 antibody and analyzed by Western blotting with SP-C antibody. (B) HEK293 cells were transfected with plasmids encoding ERdj4 or ERdj5; no cross-linking reagent was added in this experiment. Cell lysates were immunoprecipitated with p97 antibody, and then they were analyzed by Western blotting with HA antibody.