Table 2.

Quantitative immunoprecipitation analyses of ERAD substrates with ERdj4 and ERdj5^a

Substrate	ERdj4 WT:mutant	ERdj5 WT:mutant
SP-CΔexon4	1:40.50 ± 25.48	1:15.99 ± 11.27
SP-CL188Q	1:30.80 ± 21.35	1:29.26 ± 30.33
Insulin 2C96Y	1:1.18 ± 0.27	1:2.70 ± 0.73

^a HEK293 cells were transfected and subjected to immunoprecipitation analyses as described in Figure 5A. Band intensity was quantified by ImageQuant (GE Healthcare, Chalfont St. Giles, United Kingdom) analyses. The value of each WT protein was arbitrarily set to 1. The data shown are the means ± SD from at least three independent experiments.