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. 2008 Jun;19(6):2363–2372. doi: 10.1091/mbc.E07-08-0840

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© 2008 by The American Society for Cell Biology

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Figure 5. — Localization of actin-regulating proteins in arp3 and profilin mutants. (A) Immunolocalization of myosin VI (a), quail (b), and singed (c) in arp3 mutant (arp3/arp3). (B) Immunolocalization of myosin VI (d), Arp3 (e), and quail (f) in profilin mutant (chic⁷⁸⁸⁶/chic⁷⁸⁸⁶) actin cones. Actin stained with Alexa-568-phalloidin (a′–f′) is shown in the middle columns. The merged images are shown in the right columns (a″–f″). (C) Schematic drawings summarize the size and shape of the actin cone and the localization of actin-regulating proteins in wild-type, arp3, and chic (profilin) mutants. Bars, 5 μm.