Figure 5.
Wild-type and lectin-deficient Crt enhance the presentation of a specific ovalbumin peptide by H-2Kb molecules. K41 cells and K42 cells expressing the indicated Crt proteins were transiently transfected with a plasmid encoding GFP fused to ubiquitin followed by the ovalbumin-derived sequence SIINFEKL. After 24 h, cells were analyzed by flow cytometry by using mAb 25D1.16, which recognizes the SIINFEKL peptide in complex with H-2Kb molecules, followed by Alexa Fluor 642-conjugated anti-mouse Ab. Samples were gated against low, medium, and high GFP fluorescence (GFP gates 1–4) and plotted against the corresponding mean 25D1.16 fluorescence. Two separate experiments are shown and include controls lacking plasmid or in which an isotype-matched primary antibody replaced mAb 25D1.16.