Figure 1.

Identification of an Mff homologue in a Drosophila siRNA screen. (A and A′) Untransfected Drosophila S2 cells stained with MitoTracker. (B and B′) Cells transfected with Drosophila Drp1 siRNA. (C and C′) Cells transfected with CG30404 siRNA. A, B, and C are images taken with a 20× lens in the primary screen (bar, 100 μm), and A′, B′, and C′ are images taken with a 100× lens in the secondary screen (bar, 10 μm). (D) Alignment of the Drosophila CG30404 protein with Mff, which is encoded by C2ORF33. Positions of exon boundaries of the human gene, as deduced from alignment with genomic sequences, are shown below the sequence and key features of the proteins are shown above. Alternative translation start sites identified in different cDNAs are marked with asterisks. (E) Schematic drawing of domains and splice variants identified with different cDNAs encoding Mff. The numbering refers to the exons shown in the alignment in D. The National Center for Biotechnology Information shows two additional isoforms, but those are most likely incomplete or aberrant clones, because they are exceedingly short and represented by single ESTs.