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Presence of TfnR and CD4-furin on the same tubular structures induced by depletion of BIG2 and BIG1. HeLa cells stably expressing CD4-furin were mock-treated (a–a″) or treated with pools of siRNAs for BIG1 and BIG2 (b–b″) and stained for TfnR (a and b) and CD4 (a′ and b′). Merged images are shown in a″ and b″. Enlarged images of b–b″ are shown in the bottom panels.
