Figure 6.

Retrograde transport of CD4-furin with mutations within the furin cytoplasmic domain. (A) Schematic representation of the structure of CD4-furin and the primary sequence of the furin cytoplasmic domain. Substituted residues within the furin cytoplasmic domain in the mutants, YA, SA, and YA/SA, are indicated. (B) HeLa cells transiently transfected with the indicated CD4-furin construct were incubated with anti-CD4 antibody and AlexaFluor488-conjugated EGF and processed for detection of the CD4 antibody as described in the legend for Figure 4B.