FIGURE 7.

Comparison of distances from ESR experiments and molecular modeling. Interspin distances of MTS-SL-labeled b-dimers are compared that were either derived from the dipolar broadened ESR spectra (squares) or from molecular modeling the E. coli subunit b-dimer as a left-handed nonstaggered coiled-coil as described in Materials and Methods (circles) and see Fig. 3 of Wise and Vogel (preceding article). Distances stemming from ESR spectral simulations that were ≫25 Å were arbitrarily set to 30 Å. Distances up to 30 Å are given in the figure if they were reproducibly obtained from the simulations and if they also correlated well with the distances derived from molecular modeling.