FIGURE 3.

Titration of fibrillar AS with NAS probes and ThioT. Purified AS fibrils with increasing amounts of (A) 2,6-ANS, (B) 2,6-TNS, (C) bis-ANS, (D) bis-TNS, and (E) ThioT. Data correspond to the emission at the indicated wavelengths, in the presence of fibrils (squares with dots) or in buffer (circles with dots). The initial protein concentration in monomeric units was 2 μM (A), 4 μM (B), and 8 μM (C–E). The lines represent the best fits obtained using Eq. 4 and the estimated parameters are given in Table 1. Data were corrected for inner filter effects.