Aggregation kinetics of AS monitored by steady-state fluorescence. (A) Emission spectra of bis-TNS during AS aggregation. (B) Time course of AS aggregation sensed by NAS dyes and ThioT; blank (0 time value) subtracted intensities at 415 (2,6-ANS), 426 (2,6-TNS), 480 (bis-ANS), 485 (bis-TNS), and 483 nm (ThioT), respectively. Lines represent the fits obtained with Eq. 1. Dye, 10 μM; protein, ∼97 μM. Samples were run in duplicate.