TABLE 1.

Properties of different fluorescent probes upon binding to fibrillar AS

	2,6-ANS	2,6-TNS	bis-ANS	bis-TNS	ThioT
(nm)*	471	475	NA	NA	NA
(nm)	417	437	493	505	483
	1.22 ± 0.01	0.35 ± 0.01	0	0	0
	167 ± 2	169 ± 2	198 ± 3	124 ± 2	21 ± 1†
	0.61	0.64	1	0.66	—
(μM)	8.8 ± 0.5	11.7 ± 0.4	8.6 ± 0.5	11.6 ± 0.7	14.9 ± 0.4†
	0.29	0.30	0.34	0.33	0.38
‡	4.2/0.10	3.9/0.10	3.4/0.22	3.3/0.27	0.5/0.23
	9.9/0.90	10.3/0.90	7.3/0.88	7.0/0.73	2.1/0.77

NA, not applicable.

^* and are the maximum wavelengths of fluorescence emission spectrum of the unbound and bound dye, respectively.

^†Values in the range 20–90 and 1–15 μM were obtained for and respectively, for different fibril preparations.

^‡Standard deviation errors of fitted lifetimes were typically 0.1 ns for the fast component and 0.05 ns for the slow component.