TABLE 3.

Salt dependence of the TBP-DNA binding for wild-type Pyrococcus woesei and its mutants

	SK
ID*	SKpred†	SKobs‡
WT	−0.56	2.1 ± 0.3
E12A	−1.51	1.3 ± 0.3
E42Af	−1.43	0.9 ± 0.8
D101A	−1.05	2.1 ± 1.1
E128A	−1.24	1.6 ± 0.3
E12AE128A	−2.13	0.3 ± 0.2
E12AE42Af	−2.36	0.8 ± 0.2
3	−3.98	−1.1 ± 0.1
4	−4.54	−1.7 ± 0.1

^*Wild-type and PwTBP mutants. Labels 3 and 4 represent multiple mutants E12AE41KE128A and E12AE41KE42KE128A, respectively.

^†SK_pred = −dΔG_elec/dlog[salt] is obtained as the coefficient from a least-square linear fit of the computed −ΔG_elec versus the log of [salt] in the range 0.1–0.4 M.

^‡The experimental SK_obs from previous works by Ladbury and co-workers (15,21).