TABLE 1.
Partition coefficients, Stern-Volmer quenching constants, and maximal leakage, hemifusion, and fusion values at a phospholipid/peptide ratio of 15:1 for the E2FP peptide incorporated in LUVs of different compositions
| LUV compositions | KP (Trp) | Ksv (M−1) Acrylamide | Ksv (M−1) 5NS | Ksv (M−1) 16NS | Leakage (max %) | Hemifusion (max %) | Fusion (max %) |
|---|---|---|---|---|---|---|---|
| EPG/Chol, 5:1 | 4.85 × 105 | 1.124 | 4.44435 | 3.119 | 100 | 100 | 59 |
| EPA/Chol, 5:1 | 1.24 × 106 | 2.006 | 2.69487 | 1.708 | 100 | 100 | 55 |
| BPS/Chol, 5:1 | 8.6 × 105 | 3.826 | 5.65617 | 1.979 | 100 | 100 | 56 |
| EPC/Chol, 5:1 | 2.49 × 105 | 3.418 | 2.16296 | 0.550 | 78 | 69 | 50 |
| Liver extract | 2.57 × 105 | 2.782 | 3.84889 | 0.131 | 46 | 46 | 40 |
| E2FP in buffer | — | 5.456 | — | — | — | — | — |